ASSESSMENT OF ANTI-INFLAMMATORY ACTION OF VITEXIN USING IN VITRO MODELS

Abhishek Kashyap; Faizan Ali

doi:10.24092/CRPS.2026.160202

Abhishek Kashyap RB Science, Bhopal, Madhya Pradesh, India
Faizan Ali RB Science, Bhopal, Madhya Pradesh, India

DOI https://doi.org/10.24092/CRPS.2026.160202

Abstract

In the present work the in vitro anti-inflammatory potential of vitexin was evaluated using two different models of inflammation, viz., inhibition of protein denaturation and inhibition of protease action. Both these models are based on the chemical mediators of inflammation and evaluating the drug on these models is self-evident of its anti-inflammatory property. Vitexin is a natural flavanol glycoside with iron chelating and antioxidant properties. It has diverse actions at the cellular and physiological levels, demonstrating anti-inflammatory, anti-cancer, and anti-fibrosis effects in animal models. The presence of peaks at 1727, 3407, 1462 and 2842 are indicative of the presence of carbonyl, hydroxyl, aromatic ring and aliphatic carbon respectively. The results of solubility analysis indicate that vitexin is soluble in organic solvents easily and is partially or sparingly soluble in aqueous solutions. The capacity of different concentrations of vitexin to inhibit protein denaturation of albumin was ranging from 20.33 ± 0.129 % to 69.63 ± 0.213 % in the assay and hence provides yet another evidence for its excellent anti-inflammatory property. The capacity of different concentrations of vitexin to inhibit serine protease was found to be ranging from 18.12 ± 0.214% to 75.21 ± 0.315% in the assay and therefore reinforces its tremendous anti-inflammatory property.

Keywords: Vitexin, anti-inflammatory, albumin denaturation, serine protease, flavanol

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