Tulsi: A Miracle herb used in the Treatment of Many Illnesses: A Review

  • Prabha Shankar Maurya Faculty of Pharmacy, Shakti College of Pharmacy, Dulhinpur, Balrampur, India
  • Mohd. Javed Naim Faculty of Pharmacy, Bhagwant University, Ajmer, Rajasthan, India
  • Rajib K. Singh Faculty of Pharmacy, Shakti College of Pharmacy, Dulhinpur, Balrampur, India
  • Jainul Bashar Faculty of Pharmacy, Shakti College of Pharmacy, Dulhinpur, Balrampur, India
  • Radheshyam Faculty of Pharmacy, Shakti College of Pharmacy, Dulhinpur, Balrampur, India

Abstract

Tulsi, the famous "unmatched" plant of India, is one of the most popular and beneficial of the numerous therapeutic and health-promoting herbs. Ayurvedic and Unani systems Medicinal natural products are increasingly being investigated in clinical trials for superior pharmacological responses and lack of side effects compared to symptomatic agents. Ocimum sanctum, often referred to as "Holy Basil" or "Tulsi," is known in the traditional Ayurvedic literature for its use in the treatment of many illnesses. The active ingredients obtained from plants, and their biological function in disease prevention have stimulated people's curiosity. This overview includes the nomenclature of plant vesicles, their components, and their use in the treatment of diseases.

Keywords: Illness, Ayurveda, Diseases, Treat, Tulsi, Natural product, Plant

References

1. World Health Organisation. Preventing Chronic Diseases. A Vital Investment.WHO Global Report. Geneva. World Health Organization.2005. Department of Chronic Diseases and Health Promotion. p. 18.
2. Bast F, Rani P, Meena D. Chloroplast DNA phylogeography of holy basil (Ocimum tenuiflorum) in Indian subcontinent. ScientificWorldJournal. 2014;2014:847–482
3. Cohen MM. Tulsi - Ocimum sanctum.A herb for all reasons. J Ayurveda Integr Med 2014;5:251-9
4. Pattanayak, et al. A review on Ocimum sanctum Linn. A reservoir plant for therapeutic applications. An overview, Pharmacognosy Reviews, January-June 2010 Vol 4 Issue 7. Page. No. 95-105.
5. Singh N, Hoette Y, Miller R. Tulsi. The Mother Medicine of Nature. 2nd ed. Lucknow. International Institute of Herbal Medicine; 2010. pp. 28–47.
6. Mahajan N, Rawal S, Verma M, Poddar M, Alok S. A phytopharmacological overview on Ocimum species with special emphasis on Ocimum sanctum. Biomed Prev Nutr. 2013;3:185–92.
7. Mohan L, Amberkar MV, Kumari M. Ocimum sanctum linn. (TULSI)-an overview. Int J Pharm Sci Rev Res. 2011;7:51–55.
8. Pattanayak P, Behera P, Das D, Panda SK. Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacogn Rev. 2010;4:95–105.
9. Mondal S, Mirdha BR, Mahapatra SC. The science behind sacredness of Tulsi (Ocimum sanctum Linn.) Indian J Physiol Pharmacol. 2009;53:291–306.
10. Wangcharoen W, Morasuk W. Antioxidant capacity and phenolic content of holy basil. Songklanakarin J Sci Technol. 2007;29:1407–15.
11. Panda VS, Naik SR. Evaluation of cardioprotective activity of Ginkgo biloba and Ocimum sanctum in rodents. Altern Med Rev. 2009;14:161–71.
12. Shivananjappa M, Joshi M. Aqueous extract of tulsi (Ocimum sanctum) enhances endogenous antioxidant defenses of human hepatoma cell line (HepG2) J Herbs Spices Med Plants. 2012;18:331–48.
13. Manikandan P, Murugan RS, Abbas H, Abraham SK, Nagini S. Ocimum sanctum Linn. (Holy Basil) ethanolic leaf extract protects against 7,12-dimethylbenz (a) anthracene-induced genotoxicity, oxidative stress, and imbalance in xenobiotic-metabolizing enzymes. J Med Food. 2007; 10:495–502.
14. Siddique YH, Ara G, Beg T, Afzal M. Anti-genotoxic effect of Ocimum sanctum L. extract against cyproterone acetate induced genotoxic damage in cultured mammalian cells. Acta Biol Hung. 2007;58:397–409.
15. Jha A.K., Jha M, Kaur J. Ethanolic extracts of Ocimum sanctum, Azadirachta indica and Withania somnifera cause apoptosis in SiHa cells. Res J Pharm Biol Chem. 2012;3:557–62.
16. Manikandan P, Vidjaya Letchoumy P, Prathiba D, Nagini S. Combinatorial chemopreventive effect of Azadirachta indica and Ocimum sanctum on oxidant-antioxidant status, cell proliferation, apoptosis and angiogenesis in a rat forestomach carcinogenesis model. Singapore Med J. 2008;49:814–22.
17. Rastogi S, Shukla Y, Paul BN, Chowdhuri DK, Khanna SK, Das M. Protective effect of Ocimum sanctum on 3-methylcholanthrene, 7,12-dimethylbenz (a) anthracene and aflatoxin B1 induced skin tumorigenesis in mice. Toxicol Appl Pharmacol. 2007;224:228–40.
18. Shah K, Verma RJ. Protection against butyl p-hydroxybenzoic acid induced oxidative stress by Ocimum sanctum extract in mice liver. Acta Pol Pharm. 2012;69:865–70.
19. Enayatallah S.A, Shah S.N, Bodhankar S.L. A study of hepatoprotective activity of Ocimum sanctum (Krishna tulas) extracts in chemically induced liver damage in albino mice. J Ecophysiol Occup Health. 2004;4:89–96.
20. Shyamala A.C, Devaki T. Studies on peroxidation in rats ingesting copper sulphate and effect of subsequent treatment with Ocimum sanctum. J Clin Biochem Nutr. 1996;20:113–9.
21. Bawankule D.U, Pal A, Gupta S, Yadav S, Misra A, Rastogi S, et al. Protective effect of Ocimum sanctum on ethanol-induced oxidative stress in Swiss Albino Mice brain. Toxicol Int. 2008;5:121–5.
22. Verma P, Kedia D.K, Nath A. Protective effect of Ocimum sanctum leaf extracts against rogor induced ovarian toxicity in Clarias batrachus Linn. J Ecophysiology Occup Health. 2007;7:177–84.
23. Khanna A, Shukla P, Tabassum S. Role of Ocimum sanctum as a genoprotective agent on chlorpyrifos-induced genotoxicity. Toxicol Int. 2011;18:9–13.
24. Bharath BK, Anjaneyulu Y, Srilatha C. Imuuno-modulatory effect of Ocimum sanctum against endosulfan induced immunotoxicity. Vet World. 2011;4:25–7.
25. Mediratta P.K, Tanwar K, Reeta K.H, Mathur R, Benerjee B.D, Singh S, et al. Attenuation of the effect of lindane on immune responses and oxidative stress by Ocimum sanctum seed oil (OSSO) in rats. Indian J Physiol Pharmacol. 2008;52:171–7.
26. Makwana M, Rathore H.S. Prevention of hepatorenal toxicity of acetaminophen with Ocimum sanctum in mice. Int J Pharm Technol. 2011;3:1385–96.
27. Mahaprabhu R, Bhandarkar AG, Jangir B.L, Rahangadale SP, Kurkure N.V. Ameliorative effect of Ocimum Sanctum on meloxicam induced toxicity in wistar rats. Toxicol Int. 2011;18:13.1–6.
28. Lahon K, Das S. Hepatoprotective activity of Ocimum sanctum alcoholic leaf extract against paracetamol-induced liver damage in Albino rats. Pharmacognosy Res. 2011;3:1.3–8.
29. Pemminati S, Nair V, Dorababu P, Gopalakrishna H.N, Pai M.R. Effect of ethanolic leaf extract of Ocimum sanctum on haloperidol-induced catalepsy in albino mice. Indian J Pharmacol. 2007;39:87.7–9.
30. Ubaid R.S, Anantrao K.M, Jaju JB, Mateenuddin M. Effect of Ocimum sanctum (OS) leaf extract on hepatotoxicity induced by antitubercular drugs in rats. Indian J Physiol Pharmacol. 2003;47:465–70.
31. Karamala S.K, Srilatha C, Anjaneyulu Y, ChandraSekharaRao TS, Sreenivasulu D, Pidugu A.P. Hematobiochemical changes of lead poisoning and amelioration with Ocimum sanctum in wistar albino rats. Vet World. 2011;4:26.1–3.
32. Sharmila Banu G, Kumar G, Murugesan A.G. Effects of leaves extract of Ocimum sanctum L. on arsenic-induced toxicity in Wistar albino rats. Food Chem Toxicol. 2009;47:490–5.
33. Sharma M.K, Kumar M, Kumar A. Ocimum sanctum aqueous leaf extract provides protection against mercury induced toxicity in Swiss albino mice. Indian J Exp Biol. 2002;40:1079–82.
34. Bhartiya U.S, Raut Y.S, Joseph L.J. Protective effect of Ocimum sanctum L after high-dose iodine exposure in mice.An in vivo study. Indian J Exp Biol. 2006;44:647–52.
35. Joseph L.J et al. Radioprotective effect of ocimum sanctum and amifostine on the salivary gland of rats after therapeutic radioiodine exposure. Cancer Biother Radiopharm. 2011;26:737–43.
36. Reshma K et al. Tulasi (Ocimum Sanctum) as radioprotector in head and neck cancer patients undergoing radiation therapy. Biomedicine. 2012;32:39–44.
37. Singh N et al. Therapeutic Potential of Ocimum sanctum in prevention and treatment of cancer and exposure to radiation: An overview. Int J Pharm Sci Drug Res. 2012;4:97–104.
38. Uma Devi P et al . Radiation protection by the Ocimum flavonoids orientin and vicenin: Mechanisms of action. Radiat Res. 2000;154:455–60.
39. Reshma K et al. Radioprotective effects of ocimum flavonoids on leukocyte oxidants and antioxidants in oral cancer. Indian J Clin Biochem. 2008;23:171–5.
40. Bhartiya U.S et al. Effect of Ocimum sanctum, turmeric extract and vitamin E supplementation on the salivary gland and bone marrow of radioiodine exposed mice. Indian J Exp Biol. 2010;48:566–71.
41. Monga J, Sharma M, Tailor N, Ganesh N. Antimelanoma and radioprotective activity of alcoholic aqueous extract of different species of Ocimum in C (57) B.L. mice. Pharm Biol. 2011;49:428–36.
42. Uma Devi P, Ganasoundari A, Rao BS, Srinivasan KK. In vivo radioprotection by ocimum flavonoids: Survival of mice. Radiat Res. 1999;151:74–8.
43. Nayak V, Devi P.U. Protection of mouse bone marrow against radiation-induced chromosome damage and stem cell death by the ocimum flavonoids orientin and vicenin. Radiat Res. 2005;163:165–71.
44. Samson J, Sheeladevi R, Ravindran R. Oxidative stress in brain and antioxidant activity of Ocimum sanctum in noise exposure. Neurotoxicology. 2007;28:679–85.
45. Archana R, Namasivayam A. A comparative study of different crude extracts of Ocimum sanctum on noise stress. Phytother Res. 2002;16:579–80.
46. Sembulingam K, Sembulingam P, Namasivayam A. Effect of ocimum sanctum linn on changes in leucocytes of albino rats induced by acute noise stress. Indian J Physiol Pharmacol. 1999;43:137–140.
47. Sembulingam K, Sembulingam P, Namasivayam A. Effect of Ocimum sanctum Linn on the changes in central cholinergic system induced by acute noise stress. J Ethnopharmacol. 2005;96:477–82.
48. Ahmad M.Z, Ali M, Mir S.R. Anti-diabetic activity of Ocimum sanctum L. roots and isolation of new phytoconstituents using two-dimensional nuclear magnetic resonance spectroscopy. J Pharmacogn Phytother. 2012;4:75–85.
49. Singh P.K, Baxi D, Banerjee S, Ramachandran A.V. Therapy with methanolic extract of Pterocarpus marsupium Roxb and Ocimum sanctum Linn reverses dyslipidemia and oxidative stress in alloxan induced type I diabetic rat model. Exp Toxicol Pathol. 2012;64:441–8.
50. Suanarunsawat T, Songsak T. Anti-hyperglycaemic and anti-dyslipidaemic effect of dietary supplement of white Ocimum Sanctum Linnean before and after STZ-induced diabetes mellitus. Int J Diabetes Metab. 2005;13:18–23.
51. Suanarunsawat T, Ayutthaya W.D, Songsak T, Thirawarapan S, Poungshompoo S. Lipid-lowering and antioxidative activities of aqueous extracts of Ocimum sanctum L. leaves in rats fed with a high-cholesterol diet. Oxid Med Cell Longev. 2011;2011:96:20-25.
52. Dahiya K, Sethi J, Dhankhar R, Singh V et al. Effect of Ocimum sanctum on homocysteine levels and lipid profile in healthy rabbits. Arch Physiol Biochem. 2011;117:8–11.
53. Reddy S.S, Karuna R, Baskar R, Saralakumari D. Prevention of insulin resistance by ingesting aqueous extract of Ocimum sanctum to fructose-fed rats. Horm Metab Res. 2008;40:44–9.
54. Suanarunsawat T, Ayutthaya W.D, Songsak T, Rattanamahaphoom J. Anti-lipidemic actions of essential oil extracted from Ocimum sanctum L. leaves in rats fed with high cholesterol diet. J Appl Biomed. 2009;7:45–53.
55. Samak G, Rao M.S, Kedlaya R, Vasudevan D.M. Hypolipidemic efficacy of Ocimum sanctum in the prevention of atherogenesis in male albino rabbits. Pharmacologyonline. 2007;2:115–27.
56. Agrawal P, Rai V, Singh R.B. Randomized placebo-controlled, single blind trial of holy basil leaves in patients with noninsulin-dependent diabetes mellitus. Int J Clin Pharmacol Ther. 1996;34:406–9.
57. Rai V, Mani U.V, Iyer U.M. Effect of Ocimum sanctum leaf powder on blood lipoproteins, glycated proteins and total amino acids in patients with non-insulin-dependent diabetes mellitus. J Nutr Environ Med. 1997;7:113–8.
58. Devra D.K, Mathur K.C, Agrawal R.P, Bhadu I, Goyal S, Agarwal V. Effect of tulsi (Ocimum sanctum Linn) on clinical and biochemical parameters of metabolic syndrome. J Nat Remedies. 2012;12:63–7.
59. Kochhar A, Sharma N, Sachdeva R. Effect of supplementation of Tulsi (Ocimum sanctum) and Neem (Azadirachta indica) leaf powder on diabetic symptoms, anthropometric parameters and blood pressure of non insulin dependent male diabetics. Ethno-Med. 2009;3:5–9.
60. Dusane MB, Joshi B.N. Islet protective and insulin secretion property of Murraya koenigii and Ocimum tenuflorum in streptozotocin-induced diabetic mice. Can J Physiol Pharmacol. 2012;90:371–8.
61. Hannan J.M, Marenah L, Ali L, Rokeya B, Flatt P.R, Abdel-Wahab Y.H. Ocimum sanctum leaf extracts stimulate insulin secretion from perfused pancreas, isolated islets and clonal pancreatic beta-cells. J Endocrinol. 2006;189:127–36.
62. Chattopadhyay R.R. Hypoglycemic effect of Ocimum sanctum leaf extract in normal and streptozotocin diabetic rats. Indian J Exp Biol. 1993;31:891–3.
63. Gholap S, Kar A. Hypoglycaemic effects of some plant extracts are possibly mediated through inhibition in corticosteroid concentration. Pharmazie. 2004;59:876–8.
64. Kothari A, Sharma S. Evaluation of anti-inflammatory effect of fresh tulsi leaves (Ocimum Sanctum) against different mediators of inflammation in albino rats. Int J Pharm Sci Rev Res. 2012;14:119–23.
65. Fernández P.B, Figueredo Y.N, Dominguez C.C, Hernández I.C, Sanabria MLG, González R, et al. Anti-inflammatory effect of lyophilized aqueous extract of Ocinum tenuiflorum on rats. Acta Farm Bonaerense. 2004;23:92–7.
66. Thakur K, Pitre K.S. Anti-inflammatory activity of extracted eugenol from Ocimum sanctum L. leaves. Rasayan J Chem. 2009;2:472–4.
67. Singh S, Majumdar D.K. Effect of Ocimum sanctum fixed oil on vascular permeability and leucocytes migration. Indian J Exp Biol. 1999;37:1136–8.
68. Singh S, Majumdar D.K. Evaluation of antiinflammatory activity of fatty acids of Ocimum sanctum fixed oil. Indian J Exp Biol. 1997;35:380–3.
69. Singh S. Comparative evaluation of antiinflammatory potential of fixed oil of different species of Ocimum and its possible mechanism of action. Indian J Exp Biol. 1998;36:1028–31.
70. Singh S, Majumdar D.K. Anti-inflammatory and antipyretic activities of Ocimum sanctum fixed oil. Int Pharmacogn. 1995;33:288–92.
71. Kelm MA, Nair MG, Strasburg GM, DeWitt DL. Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimum sanctum Linn. Phytomedicine. 2000;7:7–13.
72. Kalabharathi H.L, Suresha R.N, Pragathi B, Pushpa V.H, Satish A.M. Anti inflammatory activity of fresh tulsi leaves (Ocimum Sanctum) in albino rats. International Journal of Pharma and Bio Sciences. 2011;2:45–50.
73. Vasudevan P, Kashyap S, Sharma S. Bioactive botanicals from basil (Ocimum sp.) J Sci Ind Res (C) 1999;58:332–8.
74. Mediratta P.K, Sharma K.K, Singh S. Evaluation of immunomodulatory potential of Ocimum sanctum seed oil and its possible mechanism of action. J Ethnopharmacol. 2002;80:15–20.
75. Hemalatha R, Babu K.N, Karthik M, Ramesh R, Kumar BD, Kumar P.U. Immunomodulatory activity and Th1/Th2 cytokine response of Ocimum sanctum in myelosuppressed swiss albino mice. Trends Med Res. 2011;6:23–31.
76. Tripathi A.K, Rajora VS, Gupta D.K, Shukla S.K. Immunomodulatory activity of Ocimum sanctum and its influence on cyclophosphamide induced immunosupression. Indian J Anim Sci. 2008;78:33–6.
77. Goel A, Singh D.K, Kumar S, Bhatia A.K. Immunomodulating property of Ocimum sanctum by regulating the IL-2 production and its mRNA expression using rat's splenocytes. Asian Pac J Trop Med. 2010;3:8–12.
78. Pavaraj M, Balasubramanian V, Baskaran S, Ramasamy P. Development of immunity by extract of medicinal plant Ocimum sanctum on common carp Cyprinus carpio (L.) Res J Immunol. 2011;4:12–8.
79. Chitra G, Krishnaveni N. Immunostimulatory effect of Ocimum sanctum leaf extract on the Indian major carp, Catla catla. Plant Archives. 2011;11:213–4.
80. Mondal S et al. Double-blinded randomized controlled trial for immunomodulatory effects of Tulsi (Ocimum sanctum Linn.)leaf extract on healthy volunteers.J Ethnopharmacol. 2011;136:452–6.
81. Ali H, Dixit S. In vitro antimicrobial activity of flavanoids of Ocimum sanctum with synergistic effect of their combined form. Asian Pac J Trop Dis. 2012;2:S396–8.
82. Singh S, Malhotra M, Majumdar DK. Antibacterial activity of Ocimum sanctum L. fixed oil. Indian J Exp Biol. 2005;43:835–7.
83. Mandal S et al. Enhancing chloramphenicol and trimethoprim in vitro activity by Ocimum sanctum Linn.(Lamiaceae) leaf extract against Salmonella enterica serovar Typhi. Asian Pac J Trop Med. 2012;5: 220–4.
84. Parag S, Vijyayshree N, Rami B, Patil B. Antibacterial activity of Ocimum sanctum Linn. and its application in water purification. Res J Chem Environ. 2010;14:46–50.
85. Farivar T.N et al. Anti tuberculosis effect of Ocimum sanctum extracts in in vitro and macrophage culture. J Med Sci. 2006;6:348–51.
86. Shokeen P, Ray K, Bala M, Tandon V. Preliminary studies on activity of Ocimum sanctum, Drynaria quercifolia, and Annona squamosa against Neisseria gonorrhoeae. Sex Transm Dis. 2005;32:106–11.
87. Sawarkar H.A, Khadabadi S.S, Mankar D.M, Farooqui I.A, Jagtap N.S. Development and biological evaluation of herbal anti-acne gel. Int J PharmTech Res. 2010;2:2028–31.
88. Yucharoen R, Anuchapreeda S, Tragoolpua Y. Anti-herpes simplex virus activity of extracts from the culinary herbs Ocimum sanctum L., Ocimum basilicum L. and Ocimum americanum L. Afri J Biotechnol. 2011;10:860–6.
89. Suzuki A et al. Leishmanicidal active constituents from Nepalese medicinal plant Tulsi (Ocimum sanctum L.) Chem Pharm Bull (Tokyo) 2009;57:245–51.
90. Saini A, Sharma S, Chhibber S. Induction of resistance to respiratory tract infection with Klebsiella pneumoniae in mice fed on a diet supplemented with tulsi (Ocimum sanctum) and clove (Syzgium aromaticum) oils. J Microbiol Immunol Infect. 2009;42:107–13.
91. Deo S.S, Inam F, Mahashabde R.P. Antimicrobial activity and HPLC fingerprinting of crude ocimum extracts. E-Journal of Chemistry. 2011;8:1430–7.
92. Balakumar S, Rajan S, Thirunalasundari T, Jeeva S. Antifungal activity of Ocimum sanctum Linn.(Lamiaceae) on clinically isolated dermatophytic fungi. Asian Pac J Trop Med. 2011;4:654–7.
93. Das J, Buragohain B, Srivastava R.B. In vitro evaluation of ocimum sanctum leaf extract against dermatophytes and opportunistic fungi. Asian J Microbiol Biotechnol Environ Sci. 2010;12:789–92.
94. Chandra R, Dwivedi V, Shivam K, Jha A.K. Detection of antimicrobial activity of Oscimum sanctum (Tulsi) and trigonella foenum graecum (Methi) against some selected bacterial and fungal strains. Res J Pharm Biol Chem Sci. 2011;2:809–13.
95. Rajamma A.J, Dubey S, Sateesha S.B, Tiwari S.N, Ghosh S.K. Comparative larvicidal activity of different species of Ocimum against Culex Quinquefasciatus. Nat Prod Res. 2011;25:1916–22.
96. Gbolade A.A, Lockwood G.B. Toxicity of Ocimum sanctum L. essential oil to Aedes aegypti Larvae and its chemical composition. J Essent Oil-Bear Plants. 2008;11:148–53.
97. Inbaneson S.J, Sundaram R, Suganthi P. In vitro antiplasmodial effect of ethanolic extracts of traditional medicinal plant Ocimum species against Plasmodium falciparum. Asian Pac J Trop Med. 2012;5:103–6.
98. Kumar S, Gupta H.P, Prasad S, Rajora V.S, Prasad J.K. Antibacterial properties of garlic and tulsi in repeat breeding crossbred cows. Indian Vet J. 2011;88:28–30.
99. Sadekar R.O, Pimprikar N.M, Bhandarkar A.G, Barmase B.S. Immunomodulating effect of Ocimum sanctum linn. Dry leaf powder on humoral immune response in poultry naturally infected with IBD virus. Indian Vet J. 1998;75:73–4.
100. Deka R.S. Influence of tulsi (Ocimum sanctum) and ashwagandha (Withania somnifera) supplementation on production of organic meat in goats. Veterinary Practitioner. 2009;10:57–9.
101. Bora D.S, Khanikor B. Selective toxicity of Ageratum conyzoides and Ocimum sanctum against Exorista sorbillans (Diptera: Tachinidae) and Antheraea ussama (Lepidoptera: Saturniidae) Natl Acad Sci Lett. 2011;34:9–14.
102. Mishra P, Mishra S. Study of antibacterial activity of ocimum sanctum extract against gram positive and gram negative bacteria. Am J Food Technol. 2011;6:336–41.
103. Kumar A, Shukla R, Singh P, Dubey N.K. Chemical composition, anti-fungal and anti-aflatoxigenic activities of Ocimum sanctum L. essential oils and its safety assessment as plant based antimicrobial. Food Chem Toxicol. 2010;48:539–43.
104. Upadhyaya S, Behera J, Tewari SN. Integrated management of foliar blast through ecofriendly formulated product, Oscext-e developed from Ocimum sanctum ethanolic extract. Arch Phytopathology Plant Protect. 2012;45:2:290–300.
105. Kumar A, Dubey N.K, Srivastavaa S. Antifungal evaluation of Ocimum sanctum essential oil against fungal deterioration of raw materials of Rauvolfia serpentina during storage. Ind Crops Prod. 2013;45:30–5.
106. Wani N.S, Bhalerao A.K, Ranaware VP, Zanje R. Formulation and evaluation of herbal sanitizer. Int J PharmTech Res. 2013;5:40–3.
107. Kukreja B.J, Dodwad V. Herbal mouthwashes-A gift of nature. Int J Pharma Bio Sci. 2012;3:46–52.
108. Malik K, Arora G, Singh I. Ocimum sanctum seeds, a natural superdisintegrant: Formulation and evaluation of fast melt tablets of nimesulide. Polim Med. 2012;42:49–59.
109. Agarwal P, Nagesh L. Comparatiive evaluation of efficacy of 0.2% Chlorhexidine, Listerine and Tulsi extract mouth rinses on salivary Streptococcus mutans count of high school children--RCT. Contemp Clin Trials. 2011;32:802–8.
110. Malhotra R, Grover V, Kapoor A, Saxena D. Comparison of the effectiiveness of a commercially available herbal mouthrinse with chlorhexidine gluconate at the clinical and patient level. J Indian Soc Periodontol. 2011;15:349–52.
111. Singh S, Taneja M, Majumdar DK. Biological activities of Ocimum sanctum L. fixed oil--an overview. Indian J Exp Biol. 2007;45:403–12.
112. Shetty S, Udupa S, Udupa L. Evaluation of antioxiidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn in rats. Evid Based Complement Alternat Med. 2008;5:95–101.
113. Goel A, Kumar S, Singh D.K, Bhatia A.K. Wound healing potentiaal of Ocimum sanctum Linn. with induction of tumour necrosis factor-alpha. Indian J Exp Biol. 2010;48:402–6.
Statistics
480 Views | 408 Downloads
How to Cite
Prabha Shankar Maurya, Mohd. Javed Naim, Rajib K. Singh, Jainul Bashar, and Radheshyam. “Tulsi: A Miracle Herb Used in the Treatment of Many Illnesses: A Review”. Current Research in Pharmaceutical Sciences, Vol. 11, no. 3, Dec. 2021, pp. 72-85, doi:10.24092/CRPS.2021.110301.