Preparation and Characterization of Polymeric Nanoparticles for Sustained Delivery of Insulin

  • Ashish Kumar Parashar Pharmaceutics Research Laboratory, Rishiraj college of Pharmacy, Indore (M.P.) 453331, India
  • Rajesh Kumar Nema Pharmaceutics Research Laboratory, Rishiraj college of Pharmacy, Indore (M.P.) 453331, India

Abstract

The present study was aimed at developing and exploring a novel sustained release formulation of PEGylated insulin encapsulated in polymeric nanoparticles that produces prolonged insulin release. The insulin was conjugated with PEG-2000 at specific amino terminus of its B chain. PEGylated insulin was encapsulated in PLGA nanoparticles made by double emulsification method. Insulin conformation and antidiabetic activity were retained after PEGylation and PLGA encapsulation. The nano-spherical particles revealed a low burst release, an important safety feature for an extended release insulin product. In PBS (pH 7.4) at 37oC, formulations with drug content of approximately 14.4% showed very low initial release of insulin over one day and near zero order drug release after a lag of 2-3 days. For animal studies, PLGA nanoparticles loaded with PEGylated insulin were administered subcutaneously as a single injection and produced a release of 15% insulin in the first day but then lowered the serum glucose levels of diabetic rats to values <200 mg/dL for approximately 2 days. Based on these findings, it is suggested that the novel PEG–insulin encapsulated PLGA nanoparticles can be used as a carrier for prolonged and sustained release insulin formulation. 

Keywords: Insulin, Nanoparticle, Poly(ethylene glycol), PLGA
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How to Cite
Parashar, A. K., and R. K. Nema. “Preparation and Characterization of Polymeric Nanoparticles for Sustained Delivery of Insulin”. Current Research in Pharmaceutical Sciences, Vol. 2, no. 3, Oct. 2012, pp. 153-9, http://crpsonline.com/index.php/crps/article/view/72.
Section
Research Articles