Development of Reverse Phase High Performance Liquid Chromatography Method for Simultaneous Estimation of Sildenafil Citrate and Depoxetine Hydrochloride in Pharmaceutical Formulation

  • Shivanand Yadav Faculty of Pharmacy, Bhagwant University, Ajmer, Rajasthan, India
  • Neeraj Sharma Faculty of Pharmacy, Bhagwant University, Ajmer, Rajasthan, India

Abstract

Simple, accurate and precise reversed-phase high-performance liquid chromatographic (RP-HPLC) methods for simultaneous estimation of sildenafil citrate (SIL) and depoxetine hydrochloride (DAP) in combined tablet dosage form have been developed and validated. The RP-HPLC method uses a Shimadzu – 1800, Software Version – UV Prob 2.33 with BDS hypersil C18 column and mixture of Buffer pH 4.0 and ACN in the ratio of 40:60 as the mobile phase. The detection was carried out using a UV–Visible Detector: Shimadzu SPD–20AT diode array detector set at 229 nm. Linearity of chromatographic method was found in the concentration range of 50 - 150 ppm for Sildenafil and 30 - 90 ppm for Dapoxetine respectively. % recovery for SIL was found to be 99.3 – 100.5 %, while for DAP it was found to be in range of 99.1 – 100.4 % in HPLC methods respectively. Both methods may be used for routine analysis of the drugs in a pharmaceutical formulation. Results of analysis were validated statistically.


 

Keywords: Linearity, Validation, HPLC, Sildenafil, Depoxetine

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How to Cite
Shivanand Yadav, and Neeraj Sharma. “Development of Reverse Phase High Performance Liquid Chromatography Method for Simultaneous Estimation of Sildenafil Citrate and Depoxetine Hydrochloride in Pharmaceutical Formulation”. Current Research in Pharmaceutical Sciences, Vol. 12, no. 1, Apr. 2022, pp. 28-31, doi:10.24092/CRPS.2022.120104.
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Research Articles