Pharmacognostic Evaluation, Phytochemical Screening and Antimicrobial Study of Leaves Extracts of UrenalobataLinn.
Urenalobatais a plant (Malvaceae family) found abundantly in tropical and sub-tropical regions. Traditionally plant is used in febrifuge, rheumatism, wound and as antiseptic. To evaluate the scientific basis for the use of the plant, the antimicrobial activities of extracts of the leaves were evaluated against some common Gram negative and Gram positive bacteria. Pulverized Urenalobataleaves were investigated for physical constant (LOD, ash value, fluorescence analysis). The fresh leaf of Urenalobatawas studied for microscopical characterization which shows upper and lower epidermis, palisade cells, spongy parenchyma in lamina while collenchyma and vascular bundles were observed in midrib. The antimicrobial activity of the ethanolic and aqueous extracts were evaluated by determination of the diameter of zone of inhibition against both Gram negative and Gram positive bacteria using agar well diffusion and minimum inhibitory concentration (MIC). Phytochemical studies revealed the presence of alkaloids, glycosides, steroids, flavonoids, tannins and both the extracts were active against Gram positive and Gram negative bacteria, of which Aqueous extract shows significant results.
2.Dixa S, Vimal S. “Urena lobata Flowers: A Green Route to Volumetric Analysis.” Green and Sustainable Chemistry, 2015; 5, 1-5.
3.Marietta C, Jeanne N. “In vitro antiproliferative and anti-oxidant activities of methanol extracts of Urena lobata and Viscum album against breast cancer cell lines.” Toxicological and environmental chemistry, 2012; 94(5), 987- 999.
4.Yudi P, Dgoko WS. “Antihyper glycemic effect of Urena lobata Leaf extact by inhibition of DPP-IV on diabetic rats.” International Journal of Pharmacognosy and Phytochemical Research, 2015; 7(5):1073-1079.
5.Keshab G, “A furocoumarin, Imperatorin isolated from Urena lobata L. (Malvaceae),” Molbank 2004, 1-2.
6.Akhere A. Omonkhua and Iyere O. Onoagbe, “Evaluation of the long-term effects of Urena lobata root extracts on blood glucose and hepatic function of normal rabbits,” Journal of Toxicology and Environmental Health Sciences, 2011; 3(8) : 204-213.
7.Thirumalaikumaran R, Chamundeeswari D, Seethalakshmi S, Gopa V. “Pharmacognostical, phytochemical and Anti oxidant studies of the aerial parts of Urena lobata L.” Basic Research Journal of Medicine and Clinical Sciences. 2013; 2(2):32-36.
8.Chatwal GR, Anand SK. Instrumental methods of chemical analysis, 7th ed., Himalaya Publishing House. 1992; 588-598.
9.Mukherjee PK, Quality control of herbal drugs, Bussiness Horizon’s, Pharmaceutical publisher, New Delhi, 2002, 138-141.
10.Evans WC, Trease and Evans, Pharmacognosy, W.B. Saunders, Edinburgh London, New York Philadelphia, 15th Edition, Pg. No.519-520, 545-547.
11.Johansen DA. Plant microtechniques, McGraw-hill Book Company, New York & London, 1st Edn 1940, 182-203.
12.Indian Pharmacopoeia, Government of India, Ministry of Health and Family Welfare, Controller of Publication, 4th edition, New Delhi, 1996. 4(II), A53-A54.
13.Pradeep KS, Mohammad A and Dinesh KY. “Physicochemical and Phytochemical evaluation of different black tea brands.” Journal of Applied Pharmaceutical Science, 2011; 01 (03):121-124.
14.Rinku M, Sanjay PU. “Antioxidant Activity of the Methanolic and Aqueous Extracts of Urena lobata (Linn.) By DPPH Method RRJPP,” Research & Reviews: Journal of Pharmacognosy and Phytochemistry, 2013; 1(1): 6-9.
15.Kokate, CK. Practical Pharmacognosy, 1st Ed, Vallabh Prakashan, New Delhi 1986, 1, 15-30.
16.Harborne, JB. Methods of extraction and isolation. In: Phytochemical methods, 3rd Chapman and Hall, London 1998, 60-66.
17.Sayyad S, Dasari B, Shaik L. “A Pharmacological Review Of Urena Lobata Plant,” Asian Journal Pharm Clin Res,2016; 9(2): 20-22.
18.Perez C, Paul M, Bazerque P. “Antibiotic assay by Agar well diffusion method,” Acta Boil Med Ex, 1990, 15, 113-115.
19.Ali MS, Faruq KA, Md. Rahman MAA. “Aslam Hossain, Antioxidant and Cytotoxic Activities of Methanol Extract of Urena lobata (L) Leaves,” hepharmajournal, 2013; l (2): 9-14.
20.Son R and Cheah YK. “Preliminary Screening Of Endophytic Fungi Frommedicinal Plants In Malaysia For Antimicrobial And Antitumor Activity,” Malaysian Journal of Medical Sciences, 2002; 9(2):23-33.
21.Mshelia IY, Dalori BM. “Effect of the Aqueous Root Extract of Urena lobata (Linn) on the Liver of Albino Rat.” Res. J. Appl. Sci., Engine. Technol., 2013; 5(1): 01-06.
22.William Kemp. Organic Spectroscopy, 3rd ed. 1991; 58-269.
23.Chatwal GR, Anand SK. Spectroscopy (Atomic ad Molecular), Himalaya Publishing House, 5th ed. 2001; 2.29-2.234
24.Sharma BK. Instrumental method of chemical analysis, 22nd ed., Goel Publishing House, Merrut. 2002; 180-224
25.Ergon Stahl. Thin Layer Chromatography, A Laboratory Hand Book, 2nd ed., New York 1990; 52-59.
26.Khamkar SP, Shelar PA.,Kakade TB, Ubale AT. “Physicochemical And Phytochemical Investigation Of Herbs Of Kas Pathar,” International Journal of Universal Pharmacy and Bio Sciences,2014; 3(6): 155-176.
27.Khamkar SP, Raskar,S. “Preliminary Phytochemical Investigation and Microbial Activity.” International Journal of Current Pharmaceutical Sciences, 2015; 1 (3):216-226.
28.Shelar PA, Jawlikar BV. “Comparative study of isolated moiety from Senna leaves, Pods and Powder.” Current Pharma Research, 2013; 3(4):1019-1022.
29.Chatwal G, R, Anand SK. Instrumental methods of chemical analysis, 7th edition, Himalaya Publishing House. 1992; 588-598.
30.Chatwal GR, Anand SK. Spectroscopy (Atomic ad Molecular), Himalaya Publishing House, 5th edition, 2001; 2.29-2.234.