Evaluation for Efficiency of Drug Delivery Systems In vitro and In vivo Plasmodium Culture using Crude Extract of Artemisia annua and artesunate
Parasitic diseases such as malaria, leishmaniasis, and trypanosomiasis represent a significant global burden and pose a great challenge to drug discovery and delivery to scientists due to their intracellular nature and disseminated locations. Moreover, poor rate of discovery in the anti-parasitic segment seen in last few decades has necessitated effective management of existing drugs by modulating their delivery. Nanoparticles (NPs) were proposed as drug carriers over 30 years ago and have received growing attention since, mainly due to their stability, enhanced loading capabilities and control over physicochemical properties. This research work emphasized on the biological and biopharmaceutical issues to be considered in the design of delivery strategy for treating plasmodium infection and role of nanoparticles in optimizing the delivery of anti-malarial drugs of natural and synthetic origin. In present study nanoparticles were prepared by using ionotropic gelation with Tpp anions ,liposomes were prepared by using thin film hydration,and phytosomes were prepared using complexing polyphenolic phytoconstituents with phosphatidylcholine.This study through in vitro and in vivo assays indicates that drug delivery system increases therapeutic index of traditional and novel drugs is enhanced via the increase of specificity due to targeting of drugs to a particular tissue, cell or intracellular compartment, the control over release kinetics, the protection of the active agent or a combination of the above.